The DNA Damage Response—Self-awareness for DNA

Radiation track and DNA damage

OGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage

Background: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (OGG1) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2’-deoxyguanosine (8-OHdG) mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this stu...

damage for organellar DNA

Our objective was to use long PCR to determine the copy number of ptDNA and mtDNA in total DNA extracted from tissue samples. In previous work, the efficiency of amplification of DNA decreased as the target sequence length increased above about 1 kb, so that DNA quantification using long PCR was considered unreliable (Arezi et al. 2003). Here, we used a long-PCR endpoint procedure to amplify or...

radiation track and dna damage

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Origins and Evaluation of DNA Damage in Infertile Individual

Assisted reproductive techniques (ART) are considered as the first line of treatment in overcoming infertility. The scope of success of these methods depends on several factors including integrity of sperm and oocyte. Obviously, paternal genomic content has an important effect on fertilization potential in infertile couples. Thus, sperm DNA damage is a genomic disorder that exists in different ...